HPLC Inline Filter and HPLC Column Frits
The HPLC inline filter (pre-column) consists of two parts, the housing and the exchangeable frit, and is not directional. For new inline filters or cleaned inline filters, they can be connected in any direction. However, if you want to understand the online filter that has been used and not cleaned, you must pay attention to the direction of use; otherwise, the filtered mechanical impurities will be washed into the chromatography system again.
Note: Depending on where the HPLC inline filter is installed in the chromatography system and how clean the mobile phase and sample are, it will need to be cleaned after a period of use. After the inline filter is contaminated, the pressure of the column gradually increases, especially when the column is removed and only the inline filter is in the system, even though the conditions under which the chromatography system is used remain unchanged.
What Should I Do If My Inline Filter is Clogged?
In general, remove the inline filter, put it in a beaker, add purified water, boil it for 5-10 minutes, and then methanol super a bit. If still blocked, take a dilute nitric acid bubble. Still can not then need to replace the new, which itself is a consumable.
Is it called an "in-line filter" or a "pre-column"?
Pre-column, as the name suggests, is a pre-column with packing inside, the retention of components, and the use of the hplc column to maintain consistency with the packing type placed in front of the column. Play two roles, one, filtration, and two, so that the impurities in the sample to the dead packing adsorption, pre-column adsorption, so that these impurities can not enter the hplc column, to protect the hplc column.
In-line filter, equivalent to a frit, the frit is neutral for the components, only to filter the role of solid particles.
Note: "Retention of components" means that the measured components will have some adhesion on the pre-column and must be washed out by the mobile phase for a while, just like the analytical column.