UHPLC Direct-connect trap 2.1mm Guard Column Hardware
The economical, leak-free cartridge design provides an unprecedented combination of convenience, economy, and reliability. The foundation of the Direct system is a reusable direct connect holder that easily attaches to any HPLC column using regular end fittings.
Hand-tight two-part PEEK/ Stainless Steel holder
Connecting the holder requires no extra tubing or fittings
Automatically adjusts to any manufacturer’s column
Perfect repeat ZDV connection
Minimal impact – no change in efficiency
The holder stays plumbed when changing cartridges
Use with uHPLCs 2.1mm Cartridges only
HPLC/UHPLC Direct Connect Guard Column Hardware provides economical protection from the harmful effects of sample and mobile phase particulates and soluble contaminants on modern HPLC and UHPLC columns. The low swept volume of the uHPLCs guard column maintains the efficiency of high performance columns assuring a negligible loss of critical resolution. With its simple design, the uHPLCs guard column installs in any analytical column in seconds and is leak tight to 18,000 PSI.
1. Why use a guard column?
- The best way to protect an analytical column from fouling is to install a guard column between the injection valve and the analytical column. The guard column traps particulates, solvent impurities and retained sample components. The ideal guard column should have no effect on the separation. In order to achieve this, a guard column with a matching bonded phase and optimal hardware configuration should be selected.
2. When should the guard column be replaced?
- The guard column should be replaced when one of the following conditions is met.
a. The theoretical number of plates of the chromatographic column is reduced by more than 15%;
b. The front pressure of the chromatographic column increases by more than 15%;
c. The resolution of the most difficult-to-separate substances decreases by more than 15%;
d. The number of sample injections exceeds 150 times;
e. The mobile phase passes through 1500 times the volume of the chromatographic column.